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KMID : 0364819870250010023
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Korean Journal of Microbiology
1987 Volume.25 No. 1 p.23 ~ p.27
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Electrophoretic Mobilities of the Potato Spindle Tuber Viroid RNA Molecules in Urea-Gradient Gels
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Lee Jai-Youl
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Abstract
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1
Low molecular weight plant ribonucleic acids including viroid-RNA molecules which are soluble in 2 M lithium chloride were electrophoresed in the 0 M to 8 M urea -gradient polyacrylamide gel. Although the linear viroid-RNA molecules migrated at a similar rate across the urea-gradient gel under the denaturing temperature, the circular viroid-RNA molecules moved more rapidly at low urea-gradient region than at high urea-gradient region. Consequently, the migration of the circular viroid-RNA molecules showed a sudden shift across the band of linear forms in the midrange of the urea-gradient gels. Electrophoretic mobilities of the circular viroid-RNA molecules seemed to depend mainly on the concentration of urea in the denaturing urea-gradient gels.
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KEYWORD
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